Result: Continuous evaluation of changes in the serum proteome from early to late stages of sepsis caused by Klebsiella pneumoniae.

Title:
Continuous evaluation of changes in the serum proteome from early to late stages of sepsis caused by Klebsiella pneumoniae.
Authors:
Raju M S; Department of Microbiology, Osmania University, Hyderabad, Telangana 500007, India., V J; Molecular Diagnostics and Biomarkers Department of Laboratory Medicine Global Hospitals, Hyderabad, Telangana 500004, India., Kamaraju RS; Molecular Diagnostics and Biomarkers Department of Laboratory Medicine Global Hospitals, Hyderabad, Telangana 500004, India., Sritharan V; Molecular Diagnostics and Biomarkers Department of Laboratory Medicine Global Hospitals, Hyderabad, Telangana 500004, India., Rajkumar K; Department of Microbiology, Osmania University, Hyderabad, Telangana 500007, India., Natarajan S; Department of Biochemistry, Osmania University, Hyderabad, Telangana 500007, India., Kumar AD; Department of Biochemistry, Osmania University, Hyderabad, Telangana 500007, India., Burgula S; Department of Microbiology, Osmania University, Hyderabad, Telangana 500007, India.
Source:
Molecular medicine reports [Mol Med Rep] 2016 Jun; Vol. 13 (6), pp. 4835-44. Date of Electronic Publication: 2016 Apr 12.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: D. A. Spandidos Country of Publication: Greece NLM ID: 101475259 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1791-3004 (Electronic) Linking ISSN: 17912997 NLM ISO Abbreviation: Mol Med Rep Subsets: MEDLINE
Imprint Name(s):
Original Publication: Athens, Greece : D. A. Spandidos
MeSH Terms:
Blood Proteins* , Klebsiella pneumoniae* , Proteome* , Proteomics*/methods, Klebsiella Infections/*blood , Sepsis/*blood, Aged ; Gene Expression Profiling ; Humans ; Klebsiella Infections/diagnosis ; Klebsiella Infections/microbiology ; Male ; Middle Aged ; Protein Interaction Mapping ; Protein Interaction Maps ; Sepsis/diagnosis ; Sepsis/microbiology ; Sepsis/mortality ; Transcriptome
Substance Nomenclature:
0 (Blood Proteins)
0 (Proteome)
Entry Date(s):
Date Created: 20160416 Date Completed: 20170406 Latest Revision: 20170406
Update Code:
20250114
DOI:
10.3892/mmr.2016.5112
PMID:
27082932
Database:
MEDLINE

Further Information

Serum protein profiles of patients with bacterial sepsis from the day of diagnosis until recovery/mortality were compared from early to late stages in response to severe sepsis using two dimensional electrophoresis. The proteins exhibiting changes during the course of sepsis (20‑28 day mortality) were selected and identified by matrix‑assisted laser desorption ionization‑time of flight‑tandem mass spectrometry. Among the proteins identified, haptoglobin (Hp), transthyretin (TTR), orosomucoid 1/α1 acid glycoprotein (ORM1), α1 antitrypsin (A1AT), serum amyloid A (SAA) and S100A9 exhibited differential expression patterns between survivors (S; n=6) and non‑survivors (NS; n=6), particularly during the early stages of sepsis. Expression factors (EFs), taken as the ratio between the NS and S during early stages, showed ratios of Hp, 0.39 (P≤0.012); TTR, 3.96 (P≤0.03); ORM1, 0.69 (P≤0.79); A1AT, 0.92 (P≤0.87) and SAA, 0.69 (P≤0.01). S100A9, an acute phase protein, exhibited an EF ratio of 1.68 (P≤0.004) during the end stages of sepsis. A delayed rise in levels was observed in Hp, A1AT, ORM1, S100A9 and SAA, whereas TTR levels increased during the early stages of sepsis in NS. Analysis of inflammatory responses in the early stages of sepsis revealed increased mRNA expression in leukocytes of interleukin (IL)‑6 (EF, 2.50), IL‑10 (EF, 1.70) and prepronociceptin (EF, 1.6), which is a precursor for nociceptin in NS compared with S, and higher Toll‑like receptor‑4 (EF, 0.30) levels in S compared with NS. Therefore, a weaker acute phase response in the early stages of sepsis in NS, combined with an inefficient inflammatory response, may contribute to sepsis mortality.