• SPEKcheck - fluorescence micro...

Treffer: SPEKcheck - fluorescence microscopy spectral visualisation and optimisation: a web application, javascript library, and data resource.

Title:
SPEKcheck - fluorescence microscopy spectral visualisation and optimisation: a web application, javascript library, and data resource.
Authors:
Phillips MA; Micron Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX13QU, UK., Susano Pinto DM; Micron Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX13QU, UK., Dobbie IM; Micron Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX13QU, UK.
Source:
Wellcome open research [Wellcome Open Res] 2018 Jul 30; Vol. 3, pp. 92. Date of Electronic Publication: 2018 Jul 30 (Print Publication: 2018).
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: Wellcome Trust Country of Publication: England NLM ID: 101696457 Publication Model: eCollection Cited Medium: Print ISSN: 2398-502X (Print) Linking ISSN: 2398502X NLM ISO Abbreviation: Wellcome Open Res Subsets: PubMed not MEDLINE
Imprint Name(s):
Original Publication: [London] : Wellcome Trust, [2016]-
References:
Wellcome Open Res. 2018 Jul 30;3:92. (PMID: 30175248)
Science. 2014 Oct 24;346(6208):1257998. (PMID: 25342811)
Nature. 2017 Jun 1;546(7656):162-167. (PMID: 28538724)
J Cell Biol. 2010 Jul 26;190(2):165-75. (PMID: 20643879)
Biophys J. 2015 Jul 7;109(1):3-6. (PMID: 26153696)
Grant Information:
United Kingdom WT_ Wellcome Trust
Contributed Indexing:
Keywords: Excitation; Fluorescence; Microscopy; Modelling
Entry Date(s):
Date Created: 20180904 Latest Revision: 20230928
Update Code:
20250114
PubMed Central ID:
PMC6107975
DOI:
10.12688/wellcomeopenres.14703.1
PMID:
30175248
Database:
MEDLINE

Weitere Informationen

Advanced fluorescence imaging methods require careful matching of excitation sources, dichroics, emission filters, detectors, and dyes to operate at their best. This complex task is often left to guesswork, preventing optimal dye:filter combinations, particularly for multicolour applications. To overcome this challenge we developed SPEKcheck, a web application to visualise the efficiency of the light path in a fluorescence microscope. The software reports values for the excitation efficiency of a dye, the collection efficiency of the emitted fluorescence, and a "brightness" score, allowing easy comparison between different fluorescent labels. It also displays a spectral plot of various elements in the configuration, enabling users to readily spot potential problems such as low efficiency excitation, emission, or high bleedthrough. It serves as an aid to exploring the performance of different dyes and filter sets.

No competing interests were disclosed.