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Treffer: Untargeted metabolomics reveals dynamic changes in metabolic profiles of rat supraspinatus tendon at three different time points after diabetes induction.

Title:
Untargeted metabolomics reveals dynamic changes in metabolic profiles of rat supraspinatus tendon at three different time points after diabetes induction.
Authors:
Xu K; Department of Sports Medicine, Affiliated Hospital of Qingdao University, Qingdao, China., Zhang L; Department of Sports Medicine, Affiliated Hospital of Qingdao University, Qingdao, China., Wang T; Department of Sports Medicine, Affiliated Hospital of Qingdao University, Qingdao, China., Ren Z; Department of Sports Medicine, Affiliated Hospital of Qingdao University, Qingdao, China., Yu T; Department of Sports Medicine, Qingdao Municipal Hospital, Qingdao, Shandong, China., Zhang Y; Department of Sports Medicine, Affiliated Hospital of Qingdao University, Qingdao, China., Zhao X; Department of Sports Medicine, Affiliated Hospital of Qingdao University, Qingdao, China.
Source:
Frontiers in endocrinology [Front Endocrinol (Lausanne)] 2023 Nov 20; Vol. 14, pp. 1292103. Date of Electronic Publication: 2023 Nov 20 (Print Publication: 2023).
Publication Type:
Journal Article; Research Support, Non-U.S. Gov't; Comment
Language:
English
Journal Info:
Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101555782 Publication Model: eCollection Cited Medium: Print ISSN: 1664-2392 (Print) Linking ISSN: 16642392 NLM ISO Abbreviation: Front Endocrinol (Lausanne) Subsets: MEDLINE
Imprint Name(s):
Original Publication: [Lausanne : Frontiers Research Foundation]
MeSH Terms:
Rotator Cuff*/chemistry , Rotator Cuff*/metabolism , Diabetes Mellitus, Type 2*/metabolism, Rats ; Animals ; Rats, Sprague-Dawley ; Uric Acid ; Metabolome
Comments:
Comment on: J Orthop Res. 2022 Apr;40(4):965-976. doi: 10.1002/jor.25112. (PMID: 34081345)
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Contributed Indexing:
Keywords: biomarker discovery; diabetes mellitus; metabolomics; progression; rotator cuff; supraspinatus
Substance Nomenclature:
268B43MJ25 (Uric Acid)
Entry Date(s):
Date Created: 20231206 Date Completed: 20231207 Latest Revision: 20240304
Update Code:
20250114
PubMed Central ID:
PMC10694349
DOI:
10.3389/fendo.2023.1292103
PMID:
38053726
Database:
MEDLINE

Weitere Informationen

Objective: To investigate the dynamic changes of metabolite composition in rat supraspinatus tendons at different stages of diabetes by untargeted metabolomics analysis.
Methods: A total of 80 Sprague-Dawley rats were randomly divided into normal (NG, n = 20) and type 2 diabetes mellitus groups (T2DM, n = 60) and subdivided into three groups according to the duration of diabetes: T2DM-4w, T2DM-12w, and T2DM-24w groups; the duration was calculated from the time point of T2DM rat model establishment. The three comparison groups were set up in this study, T2DM-4w group vs . NG, T2DM-12w group vs . T2DM-4w group, and T2DM-24w group vs . T2DM-12w group. The metabolite profiles of supraspinatus tendon were obtained using tandem mass spectrometry. Metabolomics multivariate statistics were used for metabolic data analysis and differential metabolite (DEM) determination. The intersection of the three comparison groups' DEMs was defined as key metabolites that changed consistently in the supraspinatus tendon after diabetes induction; then, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis was performed.
Results: T2DM-4w group vs. NG, T2DM-12w group vs. T2DM-4w group, and T2DM-24w group vs. T2DM-12w group detected 94 (86 up-regulated and 8 down-regulated), 36 (13 up-regulated and 23 down-regulated) and 86 (24 up-regulated and 62 down-regulated) DEMs, respectively. Seven key metabolites of sustained changes in the supraspinatus tendon following induction of diabetes include D-Lactic acid, xanthine, O-acetyl-L-carnitine, isoleucylproline, propoxycarbazone, uric acid, and cytidine, which are the first identified biomarkers of the supraspinatus tendon as it progresses through the course of diabetes. The results of KEGG pathway enrichment analysis showed that the main pathway of supraspinatus metabolism affected by diabetes (p < 0.05) was purine metabolism. The results of the KEGG metabolic pathway vs . DEMs correlation network graph revealed that uric acid and xanthine play a role in more metabolic pathways.
Conclusion: Untargeted metabolomics revealed the dynamic changes of metabolite composition in rat supraspinatus tendons at different stages of diabetes, and the newly discovered seven metabolites, especially uric acid and xanthine, may provide novel research to elucidate the mechanism of diabetes-induced tendinopathy.
(Copyright © 2023 Xu, Zhang, Wang, Ren, Yu, Zhang and Zhao.)

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.