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Treffer: STRIPAK complex defects result in pseudosexual reproduction in Cryptococcus neoformans.

Title:
STRIPAK complex defects result in pseudosexual reproduction in Cryptococcus neoformans.
Authors:
Peterson PP; Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, United States of America., Croog S; Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, United States of America., Choi Y; Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, United States of America., Sun S; Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, United States of America., Heitman J; Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, United States of America.
Source:
PLoS genetics [PLoS Genet] 2025 Jun 30; Vol. 21 (6), pp. e1011774. Date of Electronic Publication: 2025 Jun 30 (Print Publication: 2025).
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: Public Library of Science Country of Publication: United States NLM ID: 101239074 Publication Model: eCollection Cited Medium: Internet ISSN: 1553-7404 (Electronic) Linking ISSN: 15537390 NLM ISO Abbreviation: PLoS Genet Subsets: MEDLINE
Imprint Name(s):
Original Publication: San Francisco, CA : Public Library of Science, c2005-
MeSH Terms:
Cryptococcus neoformans*/genetics , Cryptococcus neoformans*/pathogenicity , Cryptococcus neoformans*/growth & development , Fungal Proteins*/genetics , Fungal Proteins*/metabolism , Reproduction, Asexual*/genetics, Gene Expression Regulation, Fungal ; Meiosis/genetics ; Signal Transduction/genetics ; Spores, Fungal/genetics ; Reproduction/genetics ; Virulence/genetics ; Genomic Instability/genetics ; Mutation
Comments:
Update of: bioRxiv. 2025 Apr 18:2025.04.08.647827. doi: 10.1101/2025.04.08.647827.. (PMID: 40297506)
References:
Microbiol Mol Biol Rev. 1998 Mar;62(1):55-70. (PMID: 9529887)
Microbiol Mol Biol Rev. 2024 Dec 18;88(4):e0020523. (PMID: 39526753)
Mol Microbiol. 2013 Nov;90(4):796-812. (PMID: 24028079)
Microbiology (Reading). 2008 Jun;154(Pt 6):1637-1645. (PMID: 18524918)
PLoS Pathog. 2024 Nov 19;20(11):e1012735. (PMID: 39561188)
Pathogens. 2020 Sep 10;9(9):. (PMID: 32927641)
Genetics. 2012 Apr;190(4):1325-39. (PMID: 22298706)
Fungal Genet Biol. 1998 Feb;23(1):110-6. (PMID: 9514693)
Mol Microbiol. 2010 Feb;75(3):658-75. (PMID: 19943902)
BMC Microbiol. 2017 Jan 19;17(1):22. (PMID: 28103800)
Cell. 2012 Jun 8;149(6):1339-52. (PMID: 22682253)
Curr Genet. 2011 Apr;57(2):133-49. (PMID: 21229248)
Fungal Genet Biol. 2000 Feb;29(1):38-48. (PMID: 10779398)
Mol Cell. 2010 Aug 27;39(4):521-34. (PMID: 20797625)
Genetics. 2022 Jan 04;220(1):. (PMID: 34791226)
Mol Cell Biochem. 2021 Feb;476(2):633-648. (PMID: 33083950)
Mol Plant Pathol. 2023 Sep;24(9):1139-1153. (PMID: 37278525)
Genetics. 2018 Apr;208(4):1357-1372. (PMID: 29444806)
Annu Rev Genet. 2019 Dec 3;53:417-444. (PMID: 31537103)
PLoS One. 2015 Apr 09;10(4):e0122916. (PMID: 25856300)
Nat Methods. 2022 Jun;19(6):679-682. (PMID: 35637307)
Fungal Genet Biol. 2010 Oct;47(10):789-99. (PMID: 20601045)
Curr Biol. 2019 Feb 4;29(3):539. (PMID: 30721675)
Nat Cell Biol. 2004 Nov;6(11):1122-8. (PMID: 15467718)
FEBS J. 2013 Jan;280(2):644-61. (PMID: 22443683)
Biol Chem. 2019 May 01;400(8):1005-1022. (PMID: 31042639)
Annu Rev Genet. 2002;36:557-615. (PMID: 12429703)
PLoS Genet. 2019 Mar 18;15(3):e1008053. (PMID: 30883543)
Mol Cell Proteomics. 2009 Jan;8(1):157-71. (PMID: 18782753)
Eukaryot Cell. 2004 Feb;3(1):232-40. (PMID: 14871953)
Mol Plant Pathol. 2016 Aug;17(6):931-42. (PMID: 26576029)
J Med Vet Mycol. 1996 Sep-Oct;34(5):299-301. (PMID: 8912162)
Cell Rep. 2016 Aug 30;16(9):2289-97. (PMID: 27545887)
Sci Rep. 2017 Dec 20;7(1):17918. (PMID: 29263343)
Infect Immun. 2009 Aug;77(8):3491-500. (PMID: 19451235)
Genetics. 2022 Jan 04;220(1):. (PMID: 34849851)
Infect Immun. 2003 Sep;71(9):4831-41. (PMID: 12933823)
Nature. 2005 Apr 21;434(7036):1017-21. (PMID: 15846346)
J Cell Sci. 2021 Jul 1;134(13):. (PMID: 34228795)
Mol Microbiol. 2012 Apr;84(2):310-23. (PMID: 22375702)
Elife. 2021 Aug 02;10:. (PMID: 34338631)
Nature. 2024 Jun;630(8016):493-500. (PMID: 38718835)
Nat Struct Mol Biol. 2021 Mar;28(3):290-299. (PMID: 33633399)
Biochem J. 2001 Feb 1;353(Pt 3):417-39. (PMID: 11171037)
BMC Biochem. 2011 Oct 10;12:54. (PMID: 21985334)
Annu Rev Microbiol. 2019 Sep 8;73:17-42. (PMID: 31082304)
PLoS Genet. 2014 Apr 17;10(4):e1004261. (PMID: 24743168)
Proc Natl Acad Sci U S A. 2023 Mar 7;120(10):e2219120120. (PMID: 36867686)
J Fungi (Basel). 2021 Jun 01;7(6):. (PMID: 34206073)
Curr Microbiol. 2000 Apr;40(4):269-73. (PMID: 10688697)
Infect Immun. 1992 Feb;60(2):602-5. (PMID: 1730495)
Microbiol Mol Biol Rev. 2000 Jun;64(2):316-53. (PMID: 10839819)
Sci Rep. 2018 May 29;8(1):8310. (PMID: 29844502)
J Biol Chem. 2018 Jul 13;293(28):11179-11194. (PMID: 29802198)
Genetics. 2003 Apr;163(4):1315-25. (PMID: 12702677)
J Cell Biol. 2017 Feb;216(2):441-461. (PMID: 28100687)
Fungal Genet Biol. 2015 May;78:65-75. (PMID: 25173822)
J Biol Chem. 2013 Jun 7;288(23):16986-16997. (PMID: 23625923)
Nature. 2007 Jan 4;445(7123):53-7. (PMID: 17086192)
Genetics. 2010 May;185(1):153-63. (PMID: 20157004)
Genetics. 1980 Mar;94(3):597-605. (PMID: 17249011)
Oncogene. 2016 Sep 1;35(35):4549-57. (PMID: 26876214)
Infect Immun. 2009 Oct;77(10):4345-55. (PMID: 19620339)
Appl Microbiol Biotechnol. 2017 Aug;101(16):6431-6445. (PMID: 28643182)
Med Mycol. 2015 Apr;53(3):225-34. (PMID: 25541555)
Grant Information:
R37 AI039115 United States AI NIAID NIH HHS; R01 AI172451 United States AI NIAID NIH HHS; R01 AI039115 United States AI NIAID NIH HHS; T32 AI052080 United States AI NIAID NIH HHS; R01 AI050113 United States AI NIAID NIH HHS
Substance Nomenclature:
0 (Fungal Proteins)
Entry Date(s):
Date Created: 20250630 Date Completed: 20250709 Latest Revision: 20250712
Update Code:
20250712
PubMed Central ID:
PMC12240305
DOI:
10.1371/journal.pgen.1011774
PMID:
40587560
Database:
MEDLINE

Weitere Informationen

STRIPAK is an evolutionarily conserved signaling complex that coordinates diverse cellular processes across fungi and animals. In the human fungal pathogen Cryptococcus neoformans, STRIPAK was recently shown to play critical roles in maintaining genome stability and controlling both sexual and asexual development. In Cryptococcus, sexual reproduction is closely linked to virulence, and our findings demonstrate that the STRIPAK complex plays key roles in both processes. Here, we further investigate the specific roles of the STRIPAK catalytic subunit Pph22 and its regulatory partner Far8 during sexual development. We show that while pph22Δ mutants are defective in α-a sexual reproduction, exhibiting impaired meiotic progression and a failure to produce viable spores, deletion of PPH22 results in exclusive pseudosexual reproduction, with progeny inheriting nuclear genomes solely from the wild-type parent. This nuclear selection appears to result from haploinsufficiency of PPH22, in which the mutant nucleus is excluded following cell-cell fusion. Overexpression of PPG1, a related phosphatase, rescued growth and developmental defects in pph22Δ mutants, and restored the preference for α-a sexual reproduction over pseudosexual reproduction during mating, suggesting functional redundancy within the STRIPAK signaling network. Furthermore, deletion of FAR8, another component of the STRIPAK complex, also led to a high rate of pseudosexual reproduction during α-a sexual mating, reinforcing the role of STRIPAK in modulating reproductive modes in C. neoformans, possibly through regulating nuclear inheritance and meiotic progression. Transcriptomic profiling of pph22Δ and far8Δ mutants revealed dysregulation of genes involved in nuclear organization, DNA replication and repair, RNA processing, cell cycle progression, and morphogenesis, suggesting that STRIPAK disruption broadly impairs cellular programs important for faithful sexual reproduction. Together, these findings highlight the distinct contributions of STRIPAK to sexual reproduction in C. neoformans and suggest that disruptions of this complex affect genome integrity and inheritance mechanisms, with broader implications for fungal adaptation and pathogenesis.
(Copyright: © 2025 Peterson et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

The authors have declared that no competing interests exist.