• Escherichia coli with a 57-cod...

Treffer: Escherichia coli with a 57-codon genetic code.

Title:
Escherichia coli with a 57-codon genetic code.
Authors:
Robertson WE; Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, UK., Rehm FBH; Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, UK., Spinck M; Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, UK., Schumann RL; Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, UK., Tian R; Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, UK., Liu W; Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, UK., Gu Y; Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, UK., Kleefeldt AA; Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, UK., Day CF; Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, UK., Liu KC; Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, UK., Christova Y; Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, UK., Zürcher JF; Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, UK., Böge FL; Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, UK., Birnbaum J; Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, UK., van Bijsterveldt L; Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, UK., Chin JW; Medical Research Council Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, UK.
Source:
Science (New York, N.Y.) [Science] 2025 Oct 23; Vol. 390 (6771), pp. eady4368. Date of Electronic Publication: 2025 Oct 23.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 0404511 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9203 (Electronic) Linking ISSN: 00368075 NLM ISO Abbreviation: Science Subsets: MEDLINE
Imprint Name(s):
Publication: : Washington, DC : American Association for the Advancement of Science
Original Publication: New York, N.Y. : [s.n.] 1880-
MeSH Terms:
Codon* , Escherichia coli*/genetics , Genes, Synthetic* , Genetic Code* , Protein Biosynthesis*, Amino Acids/genetics ; Codon, Terminator ; Genome, Bacterial
Substance Nomenclature:
0 (Amino Acids)
0 (Codon)
0 (Codon, Terminator)
Entry Date(s):
Date Created: 20250731 Date Completed: 20251023 Latest Revision: 20251118
Update Code:
20251118
DOI:
10.1126/science.ady4368
PMID:
40743368
Database:
MEDLINE

Weitere Informationen

The near-universal genetic code uses 64 codons to encode the 20 canonical amino acids and protein synthesis. Here, we designed and generated Escherichia coli with a 4-megabase synthetic genome in which we replaced known occurrences of six sense codons and a stop codon with synonymous codons. The resulting organism, Syn57, uses 55 codons to encode the 20 canonical amino acids.