• Activation of cAMP/PKA and ERK...

Treffer: Activation of cAMP/PKA and ERK signaling pathways and potential role of Phoenixin in the reproductive axis of spotted scat (Scatophagus argus)†.

Title:
Activation of cAMP/PKA and ERK signaling pathways and potential role of Phoenixin in the reproductive axis of spotted scat (Scatophagus argus)†.
Authors:
Lin X; Fisheries College, Guangdong Ocean University, Zhanjiang, China.; Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Ocean University, Zhanjiang, China., Pan Q; Fisheries College, Guangdong Ocean University, Zhanjiang, China.; Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Ocean University, Zhanjiang, China., Liu J; Fisheries College, Guangdong Ocean University, Zhanjiang, China.; Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Ocean University, Zhanjiang, China., Wu T; Fisheries College, Guangdong Ocean University, Zhanjiang, China.; Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Ocean University, Zhanjiang, China., Jiang D; Fisheries College, Guangdong Ocean University, Zhanjiang, China.; Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Ocean University, Zhanjiang, China., Shi H; Fisheries College, Guangdong Ocean University, Zhanjiang, China.; Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Ocean University, Zhanjiang, China., Tian C; Fisheries College, Guangdong Ocean University, Zhanjiang, China.; Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Ocean University, Zhanjiang, China., Chen H; Fisheries College, Guangdong Ocean University, Zhanjiang, China.; Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Ocean University, Zhanjiang, China., Li G; Fisheries College, Guangdong Ocean University, Zhanjiang, China.; Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Ocean University, Zhanjiang, China.; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture, Guangdong Ocean University, Zhanjiang, China., Deng S; Fisheries College, Guangdong Ocean University, Zhanjiang, China.; Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Ocean University, Zhanjiang, China.; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture, Guangdong Ocean University, Zhanjiang, China.
Source:
Biology of reproduction [Biol Reprod] 2026 Jan 11; Vol. 114 (1), pp. 202-214.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: Oxford University Press Country of Publication: United States NLM ID: 0207224 Publication Model: Print Cited Medium: Internet ISSN: 1529-7268 (Electronic) Linking ISSN: 00063363 NLM ISO Abbreviation: Biol Reprod Subsets: MEDLINE
Imprint Name(s):
Publication: 2017- : New York : Oxford University Press
Original Publication: Champaign, Ill. : Society for the Study of Reproduction
MeSH Terms:
Reproduction*/physiology , Reproduction*/drug effects , Cyclic AMP*/metabolism , MAP Kinase Signaling System*/physiology , MAP Kinase Signaling System*/drug effects , Cyclic AMP-Dependent Protein Kinases*/metabolism , Perciformes*/physiology , Peptide Hormones*/pharmacology , Peptide Hormones*/metabolism, Animals ; HEK293 Cells ; Male ; Humans ; Female ; Signal Transduction ; Receptors, G-Protein-Coupled/metabolism
Grant Information:
31972775 National Natural Science Foundation of China; 060302022311 Scientific Research Start-up Funds of Guangdong Ocean University; 2023A1515012880 Guangdong Basic and Applied Basic Research Foundation
Contributed Indexing:
Keywords: Scatophagus argus; HPG; Phoenixin; cAMP/PKA signaling pathway; reproductive regulation
Substance Nomenclature:
E0399OZS9N (Cyclic AMP)
EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
0 (Receptors, G-Protein-Coupled)
0 (Peptide Hormones)
Entry Date(s):
Date Created: 20250919 Date Completed: 20260116 Latest Revision: 20260116
Update Code:
20260116
DOI:
10.1093/biolre/ioaf213
PMID:
40973150
Database:
MEDLINE

Weitere Informationen

Phoenixin (Pnx) exerts its physiological function primarily through its putative receptor GPR173 in mammals. However, the signaling pathways and the function of Pnx in the regulation of reproduction in teleosts remain poorly understood. Accordingly, this study presents an investigation of the signaling pathways and their functions in the regulation of reproduction in the spotted scat (Scatophagus argus). After transfection of Gpr173a into HEK-293 T cells, the results of the dual luciferase reporter assay indicated that Pnx-14 did not alter CRE-luc or SRE-luc activity. In addition, Pnx-20 could significantly increase SRE-luc activity. After transfection with Gpr173b, Pnx-14 could significantly increase CRE-luc activity while simultaneously inhibiting SRE-luc activity. Pnx-20 could significantly enhance SRE-luc activity. In vitro hypothalamic experiments, the expression of genes regulated by Pnx-14 and Pnx-20 can be suppressed in the presence of the cAMP/PKA inhibitor H89. Pnx-20 can also significantly increase the phosphorylation level of ERK1/2. Intracerebroventricular (ICV) injection of Pnx-20 significantly elevated estradiol levels in females. Conversely, ICV administration of Pnx-14 and Pnx-20 significantly reduced 11-ketotestosterone levels in males. Furthermore, ICV injection of Pnx-14 and Pnx-20 was shown to regulate the expression of reproduction-related genes in the hypothalamus, pituitary, and gonads of both females and males. These findings suggest that Pnx-14 may activate the cAMP/PKA or ERK pathway by Gpr173b. Similarly, Pnx-20 may activate the ERK pathway by Gpr173a and Gpr173b, thereby influencing the reproductive axis in the spotted scat.
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