Treffer: Identification of an innexin required for termination of the asexual state in planarian reproductive switching.

Title:
Identification of an innexin required for termination of the asexual state in planarian reproductive switching.
Authors:
Kumagai N; Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Aomori Japan., Kuroda M; Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Aomori Japan., Hanai T; Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Aomori Japan., Fujita M; Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Aomori Japan., Hino T; Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Aomori Japan., Yorimoto S; Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Tsukuba, Japan., Manta S; Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Aomori Japan., Nakagawa S; Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Aomori Japan., Yokoyama M; Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Aomori Japan., Tajima L; Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Aomori Japan., Ito R; Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Aomori Japan., Yamada H; Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Aomori Japan., Miura K; Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Aomori Japan., Kashima M; Department of Biomolecular Science, Faculty of Science, Toho University, Funabashi, Chiba, Japan., Yamaguchi K; NIBB Core Facility, National Institute for Basic Biology, Okazaki Japan., Shigenobu S; Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Tsukuba, Japan.; NIBB Core Facility, National Institute for Basic Biology, Okazaki Japan., Furukawa R; Department of Biology, Research and Education Center for Natural Sciences, Keio University, Yokohama, Kanagawa, Japan., Sekii K; Faculty of Business and Commerce, Keio University, Yokohama, Kanagawa, Japan., Kobayashi K; Department of Biology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Aomori Japan.
Source:
PLoS genetics [PLoS Genet] 2025 Nov 18; Vol. 21 (11), pp. e1011944. Date of Electronic Publication: 2025 Nov 18 (Print Publication: 2025).
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: Public Library of Science Country of Publication: United States NLM ID: 101239074 Publication Model: eCollection Cited Medium: Internet ISSN: 1553-7404 (Electronic) Linking ISSN: 15537390 NLM ISO Abbreviation: PLoS Genet Subsets: MEDLINE
Imprint Name(s):
Original Publication: San Francisco, CA : Public Library of Science, c2005-
MeSH Terms:
Planarians*/genetics , Planarians*/physiology , Planarians*/growth & development , Reproduction, Asexual*/genetics , Helminth Proteins*/genetics , Helminth Proteins*/metabolism , Connexins*/genetics , Connexins*/metabolism, Animals ; Reproduction/genetics ; Parthenogenesis/genetics ; Male ; Female ; Germ Cells ; Pluripotent Stem Cells/metabolism
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Substance Nomenclature:
0 (Helminth Proteins)
0 (Connexins)
Entry Date(s):
Date Created: 20251118 Date Completed: 20251126 Latest Revision: 20251128
Update Code:
20251128
PubMed Central ID:
PMC12654950
DOI:
10.1371/journal.pgen.1011944
PMID:
41252438
Database:
MEDLINE

Weitere Informationen

Many metazoans switch between asexual and sexual reproduction based on environmental changes, life cycle phases, or both. This reproductive strategy enables them to benefit from the features of both reproductive modes. In general, asexual reproduction is broadly divided into parthenogenesis and vegetative reproduction. As in parthenogenesis, individuals develop ovaries and lay eggs, the most significant event in switching from parthenogenesis to sexual reproduction is the production of testes. Meanwhile, in vegetative reproduction, individuals do not need germ cells themselves. Thus, they must post-embryonically develop and maintain germ cells derived from pluripotent cells as they switch from vegetative to sexual reproduction. The complicated mechanisms for controlling the postembryonic reproductive development remain unknown. The planarian Dugesia ryukyuensis can switch from vegetative to sexual reproduction by stimulating bioactive compounds called sex-inducing substances, which are widely conserved in Platyhelminthes, including parasitic flatworms. The two reproductive modes are facilitated by the presence of adult pluripotent stem cells, which generate any type of somatic tissue in the asexual state and produce and maintain hermaphroditic reproductive organs in the sexual state. In this study, using RNA sequencing analysis in experimental sexualization by sex-inducing substances, we identified four essential genes for sexualization. A common feature following the knockdown of the four essential genes was a blockage of testicular differentiation. One of the four essential genes was a gap junction gene, Dr-siri (Dugesia ryukyuensis-sexual induction-related innexin). We suggest that the establishment of a testicular stem cell niche supported by Dr-siri protein is responsible for the breakthrough of dormancy in postembryonic reproductive development in planarian reproductive switching. Our findings suggest that the production of testes might be crucial for even switching from vegetative to sexual reproduction.
(Copyright: © 2025 Kumagai et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

The authors have declared that no competing interests exist.