• An archaeal genetic code with...

Treffer: An archaeal genetic code with all TAG codons as pyrrolysine.

Title:
An archaeal genetic code with all TAG codons as pyrrolysine.
Authors:
Kivenson V; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA., Peters SL; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA., Borrel G; Institut Pasteur, Unit Evolutionary Biology of the Microbial Cell, Paris, France., Kivenson A, Roe LT; Department of Chemistry, University of California, Berkeley, Berkeley, CA, USA., Hamlish NX; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA., Fadhlaoui K; Université Clermont Auvergne, CNRS, Laboratoire Microorganismes: Génome et Environnement, Clermont-Ferrand, France.; Université Clermont Auvergne, UMR 454 MEDIS UCA-INRAE, Clermont-Ferrand, France., Schepartz A; Department of Chemistry, University of California, Berkeley, Berkeley, CA, USA.; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.; Chan Zuckerberg Biohub, San Francisco, CA, USA.; Arc Institute, Palo Alto, CA, USA., Gribaldo S; Institut Pasteur, Unit Evolutionary Biology of the Microbial Cell, Paris, France., Hettich RL; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA., Banfield JF; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA.; Earth and Planetary Science, University of California, Berkeley, Berkeley, CA, USA.; Department of Environmental Science Policy, and Management, University of California, Berkeley, Berkeley, CA, USA.; Earth and Environmental Sciences, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.; Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.
Source:
Science (New York, N.Y.) [Science] 2025 Nov 20; Vol. 390 (6775), pp. eadu2404. Date of Electronic Publication: 2025 Nov 20.
Publication Type:
Journal Article
Language:
English
Journal Info:
Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 0404511 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9203 (Electronic) Linking ISSN: 00368075 NLM ISO Abbreviation: Science Subsets: MEDLINE
Imprint Name(s):
Publication: : Washington, DC : American Association for the Advancement of Science
Original Publication: New York, N.Y. : [s.n.] 1880-
MeSH Terms:
Archaeal Proteins*/genetics , Archaeal Proteins*/chemistry , Archaeal Proteins*/metabolism , Genetic Code* , Lysine*/analogs & derivatives , Lysine*/genetics , Lysine*/chemistry , Lysine*/analogs & derivatives , Methanosarcina*/genetics , Codon, Terminator*/genetics, Amino Acyl-tRNA Synthetases/metabolism ; Amino Acyl-tRNA Synthetases/genetics ; Escherichia coli/genetics ; Evolution, Molecular
Substance Nomenclature:
EC 6.1.1.- (Amino Acyl-tRNA Synthetases)
0 (Archaeal Proteins)
K3Z4F929H6 (Lysine)
H3214Y96LP (pyrrolysine)
0 (Codon, Terminator)
Entry Date(s):
Date Created: 20251120 Date Completed: 20251120 Latest Revision: 20251120
Update Code:
20251121
DOI:
10.1126/science.adu2404
PMID:
41264687
Database:
MEDLINE

Weitere Informationen

Multiple genetic codes developed during the evolution of eukaryotes and bacteria, yet no alternative genetic code is known for archaea. We used proteomics to confirm our prediction that certain archaea consistently incorporate pyrrolysine (Pyl) at TAG codons, supporting an alternative archaeal genetic code that we designate the Pyl code. This genetic code has 62 sense codons encoding 21 amino acids. In contrast to monophyletic genetic code distributions in bacteria, the archaeal Pyl code occurs sporadically, indicating that it arose independently in multiple lineages. We discovered that more than 1800 archaeal proteins contain Pyl, increasing the number of such proteins by two orders of magnitude. Additionally, five Pyl transfer RNA (tRNA) pyrrolysyl-tRNA synthetase pairs from Pyl-code archaea were used to introduce Pyl analogs into proteins in Escherichia coli .