Treffer: Integrating synthetic biology and process engineering for enhanced isobutanol biosynthesis yield and sustainability.
Title:
Integrating synthetic biology and process engineering for enhanced isobutanol biosynthesis yield and sustainability.
Authors:
Chen J; Department of Biochemical Engineering, University College London, Gordon Street, London, WC1H 0AH, UK., Hao J; Department of Biochemical Engineering, University College London, Gordon Street, London, WC1H 0AH, UK.; Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai, 201210, People's Republic of China.; University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China., Baganz F; Department of Biochemical Engineering, University College London, Gordon Street, London, WC1H 0AH, UK. f.baganz@ucl.ac.uk.
Source:
Biotechnology for biofuels and bioproducts [Biotechnol Biofuels Bioprod] 2026 Jan 08. Date of Electronic Publication: 2026 Jan 08.
Publication Model:
Ahead of Print
Publication Type:
Journal Article; Review
Language:
English
Journal Info:
Publisher: BioMed Central Country of Publication: England NLM ID: 9918300888906676 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2731-3654 (Electronic) Linking ISSN: 27313654 NLM ISO Abbreviation: Biotechnol Biofuels Bioprod
Imprint Name(s):
Original Publication: [London] : BioMed Central, [2022]-
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Grant Information:
NAF\R2\180721 Royal Society; 2019VCB0007 Chinese Academy of Sciences
Contributed Indexing:
Keywords: Biofuels; Bioprocessing; Biosynthesis; Engineered microorganism; Isobutanol; Metabolic engineering; Synthetic biology
Entry Date(s):
Date Created: 20260108 Latest Revision: 20260108
Update Code:
20260108
DOI:
10.1186/s13068-025-02720-8
PMID:
41501833
Database:
MEDLINE
Weitere Informationen
Isobutanol holds significant potential as a next-generation biofuel and platform chemical, offering a viable alternative to petroleum across various industries, including pharmaceuticals, and fine chemicals. Biosynthesis has emerged as a highly advantageous approach for isobutanol production, surpassing conventional chemical synthesis methods in terms of environmental sustainability, safety, and ecological compatibility. Consequently, the field of isobutanol biosynthesis has garnered increasing interest. This review provides an overview of recent breakthroughs in isobutanol biosynthesis and explores strategies aimed at improving its production yield from both biological and engineering perspectives. On the biological front, the exploration and optimization of enzymatic pathways, metabolic engineering techniques to enhance precursor availability and flux, and cellular engineering approaches to improve strain tolerance and production efficiency are meticulously analysed. Additionally, engineering aspects such as in situ product removal methods and biomass fermentation are introduced, as they significantly contribute to enhancing yields and economy. Despite the immense promise of isobutanol biosynthesis, several challenges require attention. The modification of enzymatic and metabolic pathways is crucial for increasing production efficiency and yield. The adoption of high-throughput screening techniques becomes indispensable for identifying and selecting optimal strains and enzymes with improved characteristics. Furthermore, the cytotoxic nature and volatility of isobutanol pose additional hurdles, leading to losses during the production process. By effectively addressing these challenges and advancing our understanding of isobutanol biosynthesis, we can pave the way for more efficient and sustainable production methods. This review aims to provide valuable insights and inspiration to researchers in the field, with the ultimate objective of accelerating the development and application of isobutanol biosynthesis.
(© 2025. The Author(s).)
Declarations. Competing interests: The authors declare no competing interests.