Treffer: Cloning strategy for variable antibody regions.
Title:
Cloning strategy for variable antibody regions.
Authors:
Publication Year:
2025
Subject Terms:
Biological Sciences not elsewhere classified, Information Systems not elsewhere classified, undergoes continuous manipulation, proposed design principles, present best practices, limited computational expertise, ensure necessary documentation, complex biological workflows, automated data processing, laboratory workflows, complex production, biological data, manual processing, data processing, tailored systems, single b, sample tracking, sample data, promote compliance, presented guidelines, monoclonal antibodies, modular pipeline, longer favorable, lacks scalability, laboratories run, guarantee reusability, growing acquisition, gain efficiency, extensive acquisition, experimental cycles
Document Type:
Bild
still image
Language:
unknown
Relation:
DOI:
10.1371/journal.pone.0326678.s001
Availability:
Rights:
CC BY 4.0
Accession Number:
edsbas.24259941
Database:
BASE
Weitere Informationen
After cDNA generation, three parallel PCR reactions are performed to amplify the heavy chain variable region (light blue) and either the Kappa (light green) or Lambda (pink) variable region. Upon verification by sequencing, the variable regions (V) are cloned into plasmids encoding the constant part (C) of the respective antibody chain (dark blue: heavy constant part; dark green: Kappa light constant part; purple: Lambda light constant part). See Fig 2 for a comprehensive overview. For an overview on B-cell receptor and antibody variability, refer to Fig 1 by Khatri et al. [ 51 ] and Fig 1 by Mikocziova et al. [ 52 ]. (DOCX)