Treffer: Primers used for the amplification of heavy, light Kappa (κ) and light Lambda (λ) chains.
Title:
Primers used for the amplification of heavy, light Kappa (κ) and light Lambda (λ) chains.
Authors:
Publication Year:
2025
Subject Terms:
Biological Sciences not elsewhere classified, Information Systems not elsewhere classified, undergoes continuous manipulation, proposed design principles, present best practices, limited computational expertise, ensure necessary documentation, complex biological workflows, automated data processing, laboratory workflows, complex production, biological data, manual processing, data processing, tailored systems, single b, sample tracking, sample data, promote compliance, presented guidelines, monoclonal antibodies, modular pipeline, longer favorable, lacks scalability, laboratories run, guarantee reusability, growing acquisition, gain efficiency, extensive acquisition, experimental cycles
Document Type:
dataset
Language:
unknown
DOI:
10.1371/journal.pone.0326678.s008
Availability:
Rights:
CC BY 4.0
Accession Number:
edsbas.65449F0D
Database:
BASE
Weitere Informationen
Three PCR reactions are performed per chain. PCR1 and PCR2 are performed with forward and reverse primer mixes. PCR3 is performed with specific primer pairs selected from 69 individual primers. The selection is based on sequencing results of the second PCR amplification and identification of used V(D)J alleles. See the attached Table S1.xlsx file. Refer also to Fig 2. (XLSX)