Treffer: An excerpt from the database structure explaining its relational aspects.
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Table C stores information on isolated plasmids of paired antibody chains (heavy and light), and Table D is a repository of plasmid aliquots stored as safe stocks in separate (physical) locations for future reproduction of plasmids or downstream applications. Table Ab_2 connects the information on plasmid pairs with subsequent transfection of HEK cells. Fields (i.e., columns) Working_Stock_ID, Safe_Stock_ID and Transfection_ID store unique IDs of each record per table. Fields starting with FK_ are records’ IDs inherited from related tables. Records (sample states) are connected through one-to-many relationships in the database structure; for example, a plasmid from Table C can be used for creation of a safe stock aliquot (in Table D) multiple times, while one aliquot of safe stock (in Table D) is associated with exactly one plasmid from Table C. Similarly, one transfected HEK cell sample (i.e., transfection well, in Table Ab_1) is associated with exactly two plasmids (a pair of heavy and light chain plasmids, in Table C), while this plasmid pair can be used for multiple transfections. (DOCX)