• Wet lab steps of ASCC workflow...

Treffer: Wet lab steps of ASCC workflow: thawing, expansion and differentiation of iPSCs into BMECs in a functional BBB model.

Title:
Wet lab steps of ASCC workflow: thawing, expansion and differentiation of iPSCs into BMECs in a functional BBB model.
Authors:
Malwina Kotowicz, Magdalena Shumanska, Sven Fengler, Birgit Kurkowsky, Anja Meyer-Berhorn, Elisa Moretti, Josephine Blersch, Gisela Schmidt, Jakob Kreye, Scott van Hoof, Elisa Sánchez-Sendín, S. Momsen Reincke, Lars Krüger, Harald Prüß, Philip Denner, Eugenio Fava, Dominik Stappert
Publication Year:
2025
Subject Terms:
Biological Sciences not elsewhere classified, Information Systems not elsewhere classified, undergoes continuous manipulation, proposed design principles, present best practices, limited computational expertise, ensure necessary documentation, complex biological workflows, automated data processing, laboratory workflows, complex production, biological data, manual processing, data processing, tailored systems, single b, sample tracking, sample data, promote compliance, presented guidelines, monoclonal antibodies, modular pipeline, longer favorable, lacks scalability, laboratories run, guarantee reusability, growing acquisition, gain efficiency, extensive acquisition, experimental cycles
Document Type:
Bild still image
Language:
unknown
Relation:
https://figshare.com/articles/figure/Wet_lab_steps_of_ASCC_workflow_thawing_expansion_and_differentiation_of_iPSCs_into_BMECs_in_a_functional_BBB_model_/29452358
DOI:
10.1371/journal.pone.0326678.s005
Availability:
https://doi.org/10.1371/journal.pone.0326678.s005
https://figshare.com/articles/figure/Wet_lab_steps_of_ASCC_workflow_thawing_expansion_and_differentiation_of_iPSCs_into_BMECs_in_a_functional_BBB_model_/29452358
Rights:
CC BY 4.0
Accession Number:
edsbas.DED6D15F
Database:
BASE

Weitere Informationen

Wet lab steps and timepoints (relative to the start of the differentiation – day 0: D0) are indicated by blue rectangles. Cycles of thawing (D-6), possible freezing (D-1, D6, D8), harvest and seeding (D-3, D8) of cells. Count and viability assays are carried out on D-3, D0 and D8. Media used at each timepoint are indicated by ellipses – gray: mTSER plus medium with/without rock inhibitor (RI); violet: Unconditioned Medium (UM); green: Endothelial Cell Medium with/without supplements (EC + / + , EC -/-, respectively). The TEER measurement timepoints (D10, D11) are indicated by a purple circle. For a detailed protocol, refer to Fengler et al. [ 34 ]. (DOCX)