• Sample preparation by cell gui...

Treffer: Sample preparation by cell guiding using negative dielectrophoresis

Title:
Sample preparation by cell guiding using negative dielectrophoresis
Authors:
CHRISTENSEN, Troels Balmer, PEDERSEN, Christian Møller, BANG, Dang D, WOLFF, Anders
Source:
Proceedings of the 32nd International Conference on Micro- and Nano-Engineering, Barcelona, 17-20 September 2006Microelectronic engineering. 84(5-8):1690-1693
Publisher Information:
Amsterdam: Elsevier Science, 2007.
Publication Year:
2007
Physical Description:
print, 9 ref
Original Material:
INIST-CNRS
Subject Terms:
Electronics, Electronique, Sciences exactes et technologie, Exact sciences and technology, Sciences appliquees, Applied sciences, Electronique, Electronics, Electronique des semiconducteurs. Microélectronique. Optoélectronique. Dispositifs à l'état solide, Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices, Circuits intégrés, Integrated circuits, Conception. Technologies. Analyse fonctionnement. Essais, Design. Technologies. Operation analysis. Testing, Fabrication microélectronique (technologie des matériaux et des surfaces), Microelectronic fabrication (materials and surfaces technology), Circuit intégré, Integrated circuit, Circuito integrado, Evaluation performance, Performance evaluation, Evaluación prestación, Fabrication microélectronique, Microelectronic fabrication, Fabricación microeléctrica, Lithographie UV, UV lithography, Litografía UV, Matériau conducteur, Conducting material, Material conductor, Matériau transparent, Transparent material, Material transparente, Micromachine, Micromáquina, Méthode ablation laser, Laser ablation technique, Photolithographie, Photolithography, Fotolitografía, Préparation échantillon, Sample preparation, Preparación muestreo, Système sur puce, System on a chip, Sistema sobre pastilla, Cell guiding, Dielectrophoresis, Lab on a chip, Sample pre-treatment
Document Type:
Konferenz Conference Paper
File Description:
text
Language:
English
Author Affiliations:
MIC -Department of Micro-and Nano-technology, Nano-DTU, Kgs. Lyngby 2800, Denmark
Department of Poultry, Fish and Fur Animals, Danish Institute for Food and Veterinary Research, Århus, Denmark
ISSN:
0167-9317
Access URL:
http://pascal-francis.inist.fr/vibad/index.php?action=search&terms=18807525
Rights:
Copyright 2007 INIST-CNRS
CC BY 4.0
Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS
Notes:
Electronics
Accession Number:
edscal.18807525
Database:
PASCAL Archive

Weitere Informationen

In this study, we present a microsystem designed for performing and testing dielectrophoretic (DEP) guiding of biological cells. Baker's yeast (Saccharomyces cerevisiae) is used as a model organism to study cell guiding in the system. The guiding efficiency as a function of flowrate is investigated and a decreased efficiency with increased flowrate is observed. In addition, the DEP behaviour of the yeast cells at different medium conductivities and applied frequencies is investigated. The chip is easily fabricated in a two-step process: Standard UV lithography techniques are used for electrode fabrication and laser ablation for channel structuring. The channel is sealed with an indiumtinoxide (ITO) covered polymethylmethacrylate (PMMA) lid. The ITO serves as a transparent counter electrode. This conducting and transparent lid eliminates difficult top/bottom electrode alignment and gives visual access to the microchannel.