Result: Microfluidic device for protein crystallization under controlled humidity

Title:
Microfluidic device for protein crystallization under controlled humidity
Authors:
LOUNACI, M, RIGOLET, P, ABRAHAM, C, LE BERRE, M, CHEN, Y
Source:
Proceedings of the 32nd International Conference on Micro- and Nano-Engineering, Barcelona, 17-20 September 2006Microelectronic engineering. 84(5-8):1758-1761
Publisher Information:
Amsterdam: Elsevier Science, 2007.
Publication Year:
2007
Physical Description:
print, 14 ref
Original Material:
INIST-CNRS
Subject Terms:
Electronics, Electronique, Sciences exactes et technologie, Exact sciences and technology, Physique, Physics, Domaines classiques de la physique (y compris les applications), Fundamental areas of phenomenology (including applications), Mécanique des fluides, Fluid dynamics, Mécanique des fluides appliquée, Applied fluid mechanics, Fluidique, Fluidics, Sciences appliquees, Applied sciences, Electronique, Electronics, Electronique des semiconducteurs. Microélectronique. Optoélectronique. Dispositifs à l'état solide, Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices, Circuits intégrés, Integrated circuits, Conception. Technologies. Analyse fonctionnement. Essais, Design. Technologies. Operation analysis. Testing, Fabrication microélectronique (technologie des matériaux et des surfaces), Microelectronic fabrication (materials and surfaces technology), Circuit LSI, LSI circuit, Circuito LSI, Circuit intégré, Integrated circuit, Circuito integrado, Diffraction RX, X ray diffraction, Difracción RX, Diminution coût, Cost lowering, Reducción costes, Fabrication microélectronique, Microelectronic fabrication, Fabricación microeléctrica, Lithographie, Lithography, Litografía, Microfluidique, Microfluidics, Tirage par contact, Contact printing, Tiraje por contacto, 4785N, Micro contact printing, PDMS, Protein crystallization, SAMs
Document Type:
Conference Conference Paper
File Description:
text
Language:
English
Author Affiliations:
Ecole Normale Supérieure of Paris, 24 rue Lhomond, 75005 Paris, France
Laboratoire de Biotechnologies et Pharmacologie Génétique Appliquée Ecole Normale Supérieure de Cachan, 61 av. du pdt Wilson, 94235 Cachan, France
ISSN:
0167-9317
Access URL:
http://pascal-francis.inist.fr/vibad/index.php?action=search&terms=18807541
Rights:
Copyright 2007 INIST-CNRS
CC BY 4.0
Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS
Notes:
Electronics

Physics: fluid mechanics
Accession Number:
edscal.18807541
Database:
PASCAL Archive

Further Information

Crystallization is one of the key steps for the determination of a protein structure by X-ray diffraction. Microfluidics offers new perspectives for high throughput screening of the crystallization conditions with much reduced cost. In particular, fast prototyping of microfluidic devices by soft-lithography using PDMS has lead to successful demonstrations of both on chip crystallisation and large scale integration. However, PDMS, which is gas permeable, can lead irreversibly to the liquid evaporation in microfluidic chambers. Because of the fragility of the protein crystals, this is clearly undesirable for post-crystallization experiments. We describe here a simple but yet efficient approach to solve this problem, where the PDMS device is placed inside a small tight box having two compartments separated with a thin membrane. Inside the box, a good control of humidity can be achieved and protein crystals could be conserved over a long period without dehydratation.