Treffer: VizPrimer: a web server for visualized PCR primer design based on known gene structure
College of Life Science, Sichuan University, Key Laboratory of Bio-Resources and Eco-Environment of MOE, Chengdu 610064, China
Beijing Institute of Health Service and Medical Information, Beijing 100850, China
School of Life Science, Anhui Medical University, Anhui 230032, China
CC BY 4.0
Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS
Generalities in biological sciences
Weitere Informationen
Summary: The visualization of gene structure plays an important role in polymerase chain reaction (PCR) primer design, especially for eukaryotic genes with a number of splice variants that users need to distinguish between via PCR. Here, we describe a visualized web server for primer design named VizPrimer. It utilizes the new information technology (IT) tools, HTML5 to display gene structure and JavaScript to interact with the users. In VizPrimer, the users can focus their attention on the gene structure and primer design strategy, without wasting time calculating the exon positions of splice variants or manually configuring complicated parameters. In addition, VizPrimer is also suitable for the design of PCR primers for amplifying open reading frames and detecting single nucleotide polymorphisms (SNPs). Availability: VizPrimer is freely available at http://biocompute.bmi.ac .cn/CZlab/VizPrimer/. The web server supported browsers: Chrome (>5.0), Firefox (>3.0), Safari (>4.0) and Opera (>10.0). Contact: zhangcg@bmi.ac.cn; yangyi528@vip.sina.com.