Treffer: VizPrimer: a web server for visualized PCR primer design based on known gene structure

Title:
VizPrimer: a web server for visualized PCR primer design based on known gene structure
Source:
Bioinformatics (Oxford. Print). 27(24):3432-3434
Publisher Information:
Oxford: Oxford University Press, 2011.
Publication Year:
2011
Physical Description:
print, 1/4 p
Original Material:
INIST-CNRS
Document Type:
Fachzeitschrift Article
File Description:
text
Language:
English
Author Affiliations:
Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing 100850, China
College of Life Science, Sichuan University, Key Laboratory of Bio-Resources and Eco-Environment of MOE, Chengdu 610064, China
Beijing Institute of Health Service and Medical Information, Beijing 100850, China
School of Life Science, Anhui Medical University, Anhui 230032, China
ISSN:
1367-4803
Rights:
Copyright 2015 INIST-CNRS
CC BY 4.0
Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS
Notes:
Biological sciences. Generalities. Modelling. Methods

Generalities in biological sciences
Accession Number:
edscal.25273870
Database:
PASCAL Archive

Weitere Informationen

Summary: The visualization of gene structure plays an important role in polymerase chain reaction (PCR) primer design, especially for eukaryotic genes with a number of splice variants that users need to distinguish between via PCR. Here, we describe a visualized web server for primer design named VizPrimer. It utilizes the new information technology (IT) tools, HTML5 to display gene structure and JavaScript to interact with the users. In VizPrimer, the users can focus their attention on the gene structure and primer design strategy, without wasting time calculating the exon positions of splice variants or manually configuring complicated parameters. In addition, VizPrimer is also suitable for the design of PCR primers for amplifying open reading frames and detecting single nucleotide polymorphisms (SNPs). Availability: VizPrimer is freely available at http://biocompute.bmi.ac .cn/CZlab/VizPrimer/. The web server supported browsers: Chrome (>5.0), Firefox (>3.0), Safari (>4.0) and Opera (>10.0). Contact: zhangcg@bmi.ac.cn; yangyi528@vip.sina.com.