• Fast and furious: metabolic pa...

Treffer: Fast and furious: metabolic pathways fuelling Devil facial tumour disease

Title:
Fast and furious: metabolic pathways fuelling Devil facial tumour disease
Authors:
Gérard, Anne-Lise, Pirard, Florence, Vanbeek, Caitlin, Dujon, Antoine, Schultz, Aaron, Hamede, Rodrigo, Siddle, Hannah, Thomas, Frédéric, Mckenzie, Matthew, Ziemann, Mark, Ujvari, Beata
Contributors:
Deakin University [Waurn Ponds], Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [Occitanie])-Université de Montpellier (UM), Centre de Recherches Ecologiques et Evolutives sur le Cancer (MIVEGEC-CREEC), Processus Écologiques et Évolutifs au sein des Communautés (PEEC), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [Occitanie])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [Occitanie])-Université de Montpellier (UM)-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [Occitanie])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [Occitanie])-Université de Montpellier (UM), University of Tasmania [Hobart] (UTAS), University of Southampton, Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland (UQ [All campuses : Brisbane, Dutton Park Gatton, Herston, St Lucia and other locations]), Burnet Institute [Melbourne, Victoria], ARC Linkage (LP170101105), ARC Decra (DE170101116), ARC DP (DP230100162), Save The Tasmanian Devil Eric Guiler Research Funds, Deakin University’s LES Blue Sky and SEBE Industry partnership funds, CNRS International Research Project, Morris Animal Foundation grant (D19ZO--413), Hoffmann Family, Nectar Research Cloud (supported by the NCRIS-funded Australian Research Data Commons), ANR-18-CE35-0009,TRANSCAN,ECOLOGIE ET EVOLUTION DES CANCERS TRANSMISSIBLES(2018)
Publisher Information:
CCSD, 2025.
Publication Year:
2025
Collection:
collection:IRD
collection:CNRS
collection:UNIV-MONTPELLIER
collection:ANR
collection:UM-2015-2021
collection:UM-EPE
collection:INEE-CNRS
Subject Terms:
Cholesterol biosynthesis, Pathway enrichment, Transcriptomics, Transmissible cancer, Tasmanian devil, MESH: Reactive Oxygen Species, MESH: Metabolism, MESH: Glycolysis, [SDV.CAN]Life Sciences [q-bio], Cancer
Original Identifier:
BIORXIV: 2025.03.12.642198
HAL: hal-05362872
Document Type:
E-Ressource preprint<br />Preprints<br />Working Papers
Language:
English
Relation:
info:eu-repo/semantics/altIdentifier/doi/10.1101/2025.03.12.642198
DOI:
10.1101/2025.03.12.642198
Access URL:
https://hal.science/hal-05362872
https://hal.science/hal-05362872v1/document
https://hal.science/hal-05362872v1/file/Fast_and_Furious_Metabolic_Pathways_Fuelling_Devil-1.pdf
Rights:
info:eu-repo/semantics/OpenAccess
URL: http://creativecommons.org/licenses/by/
Accession Number:
edshal.hal.05362872v1
Database:
HAL

Weitere Informationen

Devil Facial Tumour Diseases (DFTD), threatening Tasmanian devils, consist of two distinct transmissible cancers, DFT1 and DFT2, with differing origins and geographic spread. We investigated the metabolic differences between DFT1 and DFT2, examining cell viability, metabolic outputs, and bulk gene expression. Using both DFT1 and DFT2 cell lines and biopsies, we found that glycolysis, oxidative phosphorylation, glutamate metabolism and fatty acid synthesis are all essential for the survival of both tumour types. However, DFT2 exhibited higher rates of glycolysis and lactate generation compared to DFT1. This coincided with elevated ATP production, cholesterol biosynthesis and ROS generation, as well as an increased reliance on fatty acid metabolism. Furthermore, DFT2 is less metabolically adaptable than DFT1, being unable to switch to oxidative phosphorylation as DFT1 can when required. These metabolic changes in DFT2 were associated with an increased expression of metastasis genes, and in conjunction with its higher growth rate, suggests a more aggressive cancer phenotype than DFT1. These findings provide a metabolic basis for developing new treatments that are applicable to both DFT1 and DFT2, as well as aid our understanding of the epidemiology and aetiology of DFTD to guide the development of conservation strategies.