• A low-level Cdkn1c/p57kip2 exp...

Treffer: A low-level Cdkn1c/p57kip2 expression in spinal progenitors drives the transition from proliferative to neurogenic modes of division

Title:
A low-level Cdkn1c/p57kip2 expression in spinal progenitors drives the transition from proliferative to neurogenic modes of division
Authors:
Mida, Baptiste, Lehmann, Nathalie, Goïame, Rosette, Coulpier, Fanny, Bouhali, Kamal, Barbosa, Isabelle, Le Hir, Hervé, Thomas-Chollier, Morgane, Fischer, Evelyne, Morin, Xavier
Contributors:
Institut de biologie de l'ENS Paris (IBENS), Département de Biologie - ENS-PSL (IBENS), École normale supérieure - Paris (ENS-PSL), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), GenomiqueENS (Genomique ENS), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Département de Biologie - ENS-PSL (IBENS), ANR-18-CE16-0021,SYMASYM,Régulation du choix entre divisions symétriques et asymétriques pendant la neurogenèse des vertébrés(2018), ANR-10-INBS-0009,France Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010), ANR-10-LABX-0054,MEMOLIFE,Memory in living systems: an integrated approach(2010)
Source:
EMBO Reports, 2025, ⟨10.1038/s44319-025-00653-9⟩
Publisher Information:
CCSD; EMBO Press, 2025.
Publication Year:
2025
Collection:
collection:ENS-PARIS
collection:CNRS
collection:PSL
collection:ENS-PSL
collection:ANR
collection:GENOMIQUEENS
collection:FRANCE-GENOMIQUE
Subject Terms:
Neuroscience, Singlecell Transcriptomics Subject Categories Development, Cdkn1c, Modes of Division, Cell Cycle, Neurogenesis, Neurogenesis Cell Cycle Modes of Division Cdkn1c Singlecell Transcriptomics Subject Categories Development Neuroscience, [SDV]Life Sciences [q-bio]
Original Identifier:
HAL: hal-05426812
Document Type:
Zeitschrift article<br />Journal articles
Language:
English
ISSN:
1469-221X
1469-3178
Relation:
info:eu-repo/semantics/altIdentifier/doi/10.1038/s44319-025-00653-9
DOI:
10.1038/s44319-025-00653-9
Access URL:
https://hal.science/hal-05426812
https://hal.science/hal-05426812v1/document
https://hal.science/hal-05426812v1/file/s44319-025-00653-9.pdf
Rights:
info:eu-repo/semantics/OpenAccess
URL: http://creativecommons.org/licenses/by/
Accession Number:
edshal.hal.05426812v1
Database:
HAL

Weitere Informationen

During vertebrate neurogenesis, a transition from symmetric proliferative to asymmetric neurogenic divisions is critical to balance growth and differentiation. Using single-cell RNA-seq data from the chick embryonic neural tube, we identify the cell cycle regulator Cdkn1c as a key regulator of this transition. While Cdkn1 is classically associated with neuronal cell cycle exit, we show that its expression initiates at low levels in neurogenic progenitors. Functionally targeting the onset of this expression impacts the course of neurogenesis: Cdkn1c knockdown impairs neuron production by favoring proliferative symmetric divisions. Conversely, inducing a low-level Cdkn1c misexpression in self-expanding progenitors forces them to prematurely undergo neurogenic divisions. Cdkn1c exerts this effect primarily by inhibiting the CyclinD1-CDK4/6 complex and G1 phase lengthening. We propose that Cdkn1c acts as a dual driver of the neurogenic transition whose low level of expression first controls the progressive entry of progenitors into neurogenic modes of division before higher expression mediates cell cycle exit in daughter cells. This highlights that the precise control of neurogenesis regulators’ expression sequentially imparts distinct functions essential for proper neural development.